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NEB’s online tools, NEBcloner and Double Digest Finder will help guide your reaction buffer selection when setting up double digests. Setting up a Double Digestion Double digests with NEB's restriction enzymes can be set up in CutSmart Buffer. Otherwise, choose an NEBuffer that results in the most activity for both enzymes. Double Digest Finder. Use this tool to guide your reaction buffer selection when setting up double-digests, a common timesaving procedure. Choosing the right buffers will help you to avoid star activity and loss of product.

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Use NEBcloner to find the right products and protocols for each in your traditional cloning workflow, including double digestion buffers. Double Digest FAQ Spotlight Q: I would like to digest DNA with EcoRI (NEB #R0101) and XbaI (NEB #R0145) at the same time. The Double Digest Finder recommends a sequential digest, using each enzyme in its supplied NEBuffer.

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NEBuilder Assembly Tool can be used to design primers for NEBuilder HiFi DNA Assembly or Gibson Assembly reactions. NEBcloner v1.8.1. NEBcloner is a guide for selecting appropriate products and viewing protocols for steps in the cloning workflow.

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Learn more at https://www.neb.com/applications/clonin While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications. For more information about commercial rights, please contact NEB's Global Business Development team at [email protected]. While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

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Use this tool to guide your reaction buffer selection when setting up double-digests, a common timesaving procedure. Choosing the right buffers will help you to avoid star activity and loss of product. Double digestions can save you time, and this video can offer tips for how to achieve the best results. Learn more at https://www.neb.com/applications/clonin While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

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Choose a DNA, RNA, genome editing, qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry.

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